Composite

Part:BBa_K5311013:Design

Designed by: Carlos Perez de Arenaza   Group: iGEM24_UPF-Barcelona   (2024-09-26)


MG_026 + RBS cell free MJ + Cry4Ba + RoxP + His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1845
    Illegal BglII site found at 3075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 607
    Illegal NgoMIV site found at 3566
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1676
    Illegal BsaI.rc site found at 3779


Design Notes

For this composite part we have codon optimized the CDS for the Cry4Ba toxin to be more compatible with C. acnes, we also introduced the standard prefix and sufixes in our sequence to ensure a proper alignment for the Gibson Assembly


Source

This part was created by using the corresponding sequences on this registry: MG26: Part: BBa_K5311000; RBS cell free MJ: Part:BBa_K5311004 ; Cry4Ba: Part:BBa_K5311003; RoxP: Part:BBa_K5311001; and the His-tag corresponds to a sequence of 6 Hystidines

References